Home
HelpTest ID: EEEVP Red Blood Cell (RBC) Enzyme Evaluation
Reporting Name
RBC Enzyme EvaluationUseful For
Identifying defects of red cell enzyme metabolism
Evaluating patients with hemolytic anemia
Clinical Information
All enzyme defects, including erythrocyte enzyme errors, are inherited; some are sex-linked and located on the X chromosome. Some family members have no hematologic abnormalities, while others have a hemolytic anemia. For a number of RBC enzyme defects (eg, deficiencies of hexokinase, glucose phosphate isomerase, pyruvate kinase), the sole clinical manifestation is hemolytic anemia. Glucose-6-phosphate dehydrogenase deficiency is the most common metabolic error of the red cell and presents with acute hemolytic anemia in response to oxidant stress (eg, drugs, acute infections, fava bean ingestion).
This is a consultative evaluation looking at red cell enzyme defects as the cause for early red cell destruction.
Interpretation
A hematopathologist expert in these disorders evaluates the case, appropriate tests are performed, and an interpretive report is issued.
Testing Algorithm
This is a consultative evaluation in which the case will be evaluated at Mayo Clinic Laboratories, the appropriate tests performed at an additional charge, and the results interpreted.
Note: RBCE / Reflexed RBC Enzymes, Blood includes: adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5'nucleotidase.
See Benign Hematology Evaluation Comparison in Special Instructions.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| EEEV | Erythrocyte Enzyme Interpretation | No | Yes |
| G6PD | G-6-PD, QN, RBC | Yes | Yes |
| PK | Pyruvate Kinase, RBC | Yes | Yes |
| GPI | Glucose Phosphate Isomerase, B | Yes | Yes |
| HEXK | Hexokinase, B | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| GLTI | Glutathione, B | No | No |
| RBCE | Reflexed RBC Enzymes | No | No |
Special Instructions
Analytic Time
2-10 days (not reported Saturday or Sunday)Day(s) and Time(s) Performed
Monday through Friday; Varies
Clinical Reference
Beutler E: Glucose-6-phosphate dehydrogenase deficiency and other enzyme abnormalities. In Hematology. Fifth edition. Edited by E Beutler, MA Lichtmann, BS Coller, TJ Kipps. New York, McGraw-Hill Book Company, 1995, pp 564-581
Method Name
EEEV: Consultative Interpretation
G6PD, GPI, PK, GLTI, HEXK, RBCE: Kinetic Spectrophotometry (KS)
Specimen Type
Whole Blood ACD-BSpecimen Required
Container/Tube: Yellow top (ACD solution B)
Specimen Volume: 12 mL
Collection Instructions: Do not transfer blood to other containers.
Specimen Minimum Volume
5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood ACD-B | Refrigerated | 8 days |
Reference Values
Definitive results and an interpretive report will be provided.
Test Classification
See Individual Test IDsCPT Code Information
82955-G-6-PD
84087-Glucose phosphate isomerase
84220-Pyruvate kinase
82657-Hexokinase
82978-Glutathione (if appropriate)
83915-RBC Enzymes (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EEEVP | RBC Enzyme Evaluation | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 21065 | Erythrocyte Enzyme Interpretation | 59466-3 |
| G6PD_ | G-6-PD, QN, RBC | 32546-4 |
| GPI_ | Glucose Phosphate Isomerase, B | 44050-3 |
| HEXK_ | Hexokinase, B | 49216-5 |
| PK_ | Pyruvate Kinase, RBC | 32552-2 |
Forms
1. Metabolic Hematology Patient Information (T810) is available in Special Instructions
2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.
mml-bone-minerals