Test ID: CHLE Cholesteryl Esters, Serum
Reporting Name
Cholesteryl Esters, SUseful For
Establishing a diagnosis of lecithin-cholesterol acyltransferase deficiency
Evaluating the extent of metabolic disturbance by bile stasis or liver disease
Clinical Information
Cholesterol in the blood serum is normally 60% to 80% esterified with fatty acids, largely as a result of the action of the enzyme lecithin-cholesterol acyltransferase (LCAT), which circulates in the blood in association with the high-density lipoproteins.
Familial deficiency of LCAT is uncommon, usually occurring in individuals of northern European descent, and is associated with erythrocyte abnormalities (target cells) and decreased (20% or less) esterification of serum cholesterol. LCAT deficiency is associated with early atherosclerosis, corneal opacification, hyperlipidemia, and mild hemolytic anemia. In persons who are deficient in LCAT, a much smaller percentage of the serum cholesterol is esterified. Persons who have a familial deficiency of LCAT have only 20% or less of serum cholesterol esterified. In association with a deficiency of LCAT, the concentration of unesterified cholesterol in the serum may increase 2 to 5 times the normal value and the concentration of lecithin may also increase.
Persons with liver disease may have impaired formation of LCAT and, therefore an acquired LCAT deficiency and reduced cholesterol ester.
Interpretation
Persons who have a familial deficiency of lecithin-cholesterol acyltransferase have only 20% or less of serum cholesterol esterified.
Report Available
1 to 3 daysDay(s) Performed
Monday through Friday
Clinical Reference
1. Meikle PJ, Mundra PA, Wong G, et al. Circulating lipids are associated with alcoholic liver cirrhosis and represent potential biomarkers for risk assessment. PLoS One. 2015;10(6):e0130346. doi:10.1371/journal.pone.0130346
2. Leach NV, Dronca E, Vesa SC, et al. Serum homocysteine levels, oxidative stress and cardiovascular risk in non-alcoholic steatohepatitis. Eur J Intern Med. 2014;25(8):762-767. doi:10.1016/j.ejim.2014.09.007
3. Santamarina-Fojo S, Hoeg JM, Assmann G, Brewer B. Lecithin cholesterol acyltransferase deficiency and fish eye disease. In: Valle DL, Antonarakis S, Ballabio A, Beaudet AL, Mitchell GA. eds. The Online Metabolic and Molecular Bases of Inherited Disease. McGraw-Hill; 2019. Accessed June 8, 2021. Available at https://ommbid.mhmedical.com/content.aspx?sectionid=225539713&bookid=2709
Method Name
Enzymatic Colorimetric
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial. Send refrigerated.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Frozen | 60 days | ||
Ambient | 24 hours |
Reference Values
≥18 years: 60-80% of total cholesterol
Reference values have not been established for patients who are less than 18 years of age.
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84311
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
CHLE | Cholesteryl Esters, S | 21197-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
CHLES | Cholesteryl Esters, S | 21197-9 |
Forms
If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.
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